Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
SIGMA/A0418 - IgG fraction of antiserum
Synonym: Goat Anti-Rabbit IgG (whole molecule)–AP
MDL Number: MFCD00162782
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:40 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A0418).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1:100 Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. M7939). The antibody was developed using Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A0418). Lanes 1. 1:100,000 antibody 2. 1:200,000 antibody 3. Negative Control
ELISA Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A0418) was serially diluted to detect Rabbit IgG by direct ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:20,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 | |
| western blot (chemiluminescent): 1:100,000 |
| Application: | Anti-Rabbit IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat has been used in western blotting and enzyme-linked immunosorbent assay (ELISA). |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6). |
| Biochem/physiol Actions: | IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region contributes for the development of therapeutic antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively. |
| General description: | Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains, held together by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. |
| Immunogen: | pooled normal rabbit serum |
| Legal Information: | CDP-Star is a registered trademark of Tropix, Inc. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide |
| Specificity: | Goat polyclonal anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody reacts with rabbit IgG in vitro, in rabbit serum and biological fluids. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |