Anti-Actin (plant) antibody, Mouse monoclonal
SIGMA/A0480 - clone 10-B3 (MAbGPa), purified from hybridoma cell culture
Synonym: Monoclonal Anti-Actin (plant) antibody produced in mouse
MDL Number: MFCD00145889
Product Type: Chemical
Western Blotting JOURNAL CITATION: Analysis of papaya cell wall-related genes during fruit ripening indicates a central role of polygalacturonases during pulp softening. By: Fabi, J. P., Broetto, S. G., et al. in PLoS One, 2014. PubMed ID: 25162506 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0105685), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Arabidoposis suspension cells labeled with the Monoclonal Anti-Actin, clone 10-B3 (MAbGPa) (Cat. No. A0480) at a 1:100 dilution. The actin filaments were revealed with Anti-Mouse IgG-FITC (Cat. No. F6257) (green) and the nucleus is stained with DAPI (red). From M.K. Kandasamy, Genetics Department, University of Georgia, Athens, GA.
Immunoblotting Plant leaves lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Actin (Plant) Clone:10-B3 (MAbGPa) (Cat. No. A0480). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Arabidopsis 2. Tobacco 3. Maize 4. Tomato
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 10-B3 (MAbGPa), monoclonal |
| conjugate | unconjugated |
| isotype | IgG2b |
| mol wt | antigen ~45 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | plant |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using corn leaves extracts |
| Application: | Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in: • two-layer phos-tag polyacrylamide gel electrophoresis (PAGE) • immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). • immunoblotting |
| Biochem/physiol Actions: | In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques. Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12). |
| General description: | Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class. |
| Immunogen: | ACT8 protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |