α-Amylase from human saliva
SIGMA/A0521 - Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
Synonym: 1,4-α-D-Glucan-glucanohydrolase
CAS Number: 9000-90-2
EC Number: 232-565-6
MDL Number: MFCD00081319
Product Type: Chemical
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| composition | Protein, 5.0-15.0% biuret |
| form | lyophilized powder |
| greener alternative category | Enabling  , |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | 1,000-3,000 units/mg protein |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| type | Type IX-A |
| UniProt accession no. | P0DUB6  |
| Application: | α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO). |
| Biochem/physiol Actions: | α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles . |
| General description: | α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva. |
| Other Notes: | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 100, 500, 2500 units in glass bottle |
| Physical form: | Lyophilized powder containing (NH4)2SO4 and sodium citrate |
| Preparation Note: | Chromatographically purified |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-24-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1,000-3,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |