Monoclonal Anti-Angiopoietin-1 antibody produced in mouse
SIGMA/A0604 - clone 171718, purified immunoglobulin
Synonym: Anti-ANG-1
MDL Number: MFCD06798694
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 171718, monoclonal |
| conjugate | unconjugated |
| isotype | IgG2b |
| Quality Level | 100  |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 25 μg/mL using cells and tissue sections |
| indirect ELISA: 0.5-1.0 μg/mL | |
| western blot: 1-2 μg/mL | |
| UniProt accession no. | Q15389  |
| Application: | Anti-Angiopoietin-1 antibody may be used for immunoblotting at a working concentration of 1-2 μg/mL. It has also been used for immunoblotting using skeletal muscle, kidney, lung, epididymal fat and brain tissue of mouse. For ELISA, the recommended concentration is 0.5-1.0 μg/mL. The antibody is suitable for immunohistochemistry at a working antibody concentration of 25 μg/mL.Monoclonal Anti-Angiopoietin-1 antibody produced in mouse has been used in Western blotting. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Angiopoietin 1 is an endothelial cell-specific protein that is a ligand for the TIE2 tyrosine receptor. It is required for mature blood vessel formation . Angiopoietins are growth factors vital for vascular differentiation during normal development. There are four angiopoietins discovered that bind to receptor tyrosine kinases Tie2. Angiopoietin-1 (ANG-1) is a potent pro-angiogenic factor that works by recruits mural cells for endothelial tubule formation and vessel maturation. ANG-1 acts along with VEGF in enhance angiogenesis and increase vascular branching. ANG-1 also affects the inflammatory process and inhibits the capillary transmigration of leukocytes on TNF-α stimulation. Sonic hedgehog, a secreted growth factor, reportedly upregulates the expression of ANG-1. Overexpression of angiopoietins has been observed in a variety of malignancies including neuroblastoma, glioblastoma and lung cancers. While ANG-2 increases the metastasis and angiogenesis of the tumors, ANG-1 inhibits tumor angiogenesis |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Angiopoietin-1 detects recombinant human angiopoietin-1. The antibody shows no cross-reactivity with recombinant human angiopoietin-2, recombinant human angiopoietin-4, recombinant mouse angiopoietin-like 3, and recombinant human cornea-derived transcript 6 (CDT-6).The ANG-1 gene is mapped to human chromosome 8q23.1. |
| Immunogen: | purified, NSO-derived, recombinant human ANG-1. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |