Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
SIGMA/A0705 - affinity isolated antibody
Synonym: Rabbit Anti-Bovine IgG (whole molecule)−AP
MDL Number: MFCD00162168
Product Type: Chemical
ELISA
Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A0705) was serially diluted to detect Bovine IgG by direct ELISA.
Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in Rabbit (Cat. No. A0705) was serially diluted to detect Bovine IgG by direct ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | bovine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:30,000 |
| Application: | Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in • enzyme linked immunosorbent assay (ELISA) • development of immunosensors • western blot analysis |
| Biochem/physiol Actions: | Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin . Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulins (Igs) belongs to immunoglobulin super-family (IgSF). Igs in general, consist of two heavy (H) chains and two light (L) chains. The L chain can be either κ or a λ chain. Each chain contains one NH2-terminal “variable" (V) region and one or more COOH-terminal “constant” (C) regions. Each of which consists of two sandwiched β pleated sheets ′pinned′ together by a disulfide bridge. There are five main classes of Igs based on heavy chain C domains, they are, IgM, IgG, IgA, IgD, and IgE isotypes. IgG can be divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. Each subclass possesses distinct biologic properties. |
| Immunogen: | Purified bovine IgG |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |