Anti-Auxin antibody, Mouse monoclonal
SIGMA/A0855 - clone 1E11-C11, purified from hybridoma cell culture
Synonym: Anti-IAA; Anti-Indole-3-acetic acid
MDL Number: MFCD07785405
Product Type: Chemical
ELISA Monoclonal Anti-Auxin Clone: 1E11-C1 (Cat. No. A0855) was serially diluted for indirect ELISA using IAA conjugated to BSA as a substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1E11-C11, monoclonal |
| conjugate | unconjugated |
| form | PBS solution |
| isotype | IgG2b |
| Quality Level | 200  |
| recombinant | expressed in mouse cell line |
| shipped in | dry ice |
| species reactivity | plant |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA | |
| western blot: suitable |
| Application: | Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody. |
| Application: | Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA. |
| Immunogen: | indoleacetic acid (IAA) carboxyl linked to ovalbumin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |