Anti-ATG5 (N-terminal) antibody produced in rabbit
SIGMA/A0856 - affinity isolated antibody, PBS solution
Synonym: ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit; Atg5 Antibody; Anti-APG5; Anti-APG5L; Anti-ASP; Anti-ATG5 autophagy related 5 homolog (S. cerevisiae); Anti-
MDL Number: MFCD09753847
Product Type: Chemical
Immunofluorescence Anti-ATG5 (N-terminal): Cat. No. A0856: Endogenous Atg5-positive neurons and their projections are highlighted by immunofluorescent labeling (red) in the central nervous system of feeding stage Drosophila larvae. Atg5 accumulates in the nucleus (labeled by DAPI in blue) of nearby gastric caeca cells expressing an Actin-GFP reporter. Submitted by Dr G. Juhasz, Eotvos Lorand University.
Immunoblotting Human K562 cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-ATG5 (N-terminal) (Cat. No. A0856). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. The antibody recognizes the Atg5-Atg12 complex. Lanes 1: Antibody 0.1 μg/mL 2: Antibody 0.2 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | PBS solution |
| mol wt | antigen ~56 kDa (Atg5-Atg12 complex) |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells | |
| UniProt accession no. | Q9H1Y0  |
| Application: | Anti-ATG5 (N-terminal) antibody has been used: • in immunoblotting • in immunofluorescence • in western blotting |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5. |
| Application: | Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses. |
| Biochem/physiol Actions: | Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |