Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
SIGMA/A1047 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (γ-chain specific)−AP
MDL Number: MFCD00162632
Product Type: Chemical
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| recombinant | expressed in goat |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:7,000-1:21,000 |
| western blot: suitable |
| Application: | Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody. |
| Application: | For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies. |
| Application: | Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific). |
| Immunogen: | purified mouse IgG |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |