Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A1054 - clone DI-22, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Digoxin; Monoclonal Anti-
Product Type: Chemical
ELISA Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody produced in Mouse, Clone: DI-22 (Cat. No. A1054) was serially diluted to detect Digoxin by direct ELISA. Reactivity is observed only with Digoxin-BSA and Digoxigenin-Transferrin but not with Transferrin and BSA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DI-22, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| recombinant | expressed in mouse cell line |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate |
| direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate | |
| dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent) |
| Application: | Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA). |
| Application: | Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin. |
| Immunogen: | Digoxin-KLH. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide |
| Specificity: | Antibody is specific for digoxin and shows a high affinity for digoxigenin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |