Anti-ATM antibody,Mouse monoclonal
SIGMA/A1106 - clone MAT3-4G10/8, purified from hybridoma cell culture
Synonym: Anti-
MDL Number: MFCD01321740
Product Type: Chemical
Western Blotting JOURNAL CITATION: Exosomes maintain cellular homeostasis by excreting harmful DNA from cells. By: Takahashi, A., Okada, R., et al. in Nat Commun, 2017. PubMed ID: 28508895 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms15287), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Total cell extract of HEK-293T cells were incubated with Monoclonal Anti-ATM (0.5 μg/mL down to 0.125 μg/mL) (Cat. No. A1106). The antibody was developed using Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 0.50 μg/mL antibody 2: 0.25 μg/mL antibody 3: 0.125 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MAT3-4G10/8, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | PBS solution |
| isotype | IgG1 |
| mol wt | antigen ~300 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| recombinant | expressed in mouse cell line |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 0.1-0.2 μg/mL using HEK-293T total cel extract | |
| UniProt accession no. | Q13315  |
| Application: | Monoclonal Anti-ATM antibody can be used in immunoblotting , ELISA and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-Ataxia-Telangiectasi |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ATM (mouse IgG1 isotype) is derived from the hybridoma MAT3-4G10/8 produced by the fusion of mouse myeloma cells (NSO) and splenocytes from BALB/c mice immunized with a peptide. |
| Immunogen: | peptide spanning positions 1967-1988 of mouse ATM containing a cysteine at its NH2 terminus coupled to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | Monoclonal Anti-ATM antibody reacts specifically with mouse and human ATM. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |