DMT-dA(bz) Pharmadite®
SIGMA/A11100P
Synonym: Adenosine, N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxy-, 3′-[2-cyanoethyl N,N-
CAS Number: 98796-53-3
Empirical Formula (Hill Notation): C47H52N7O7P
Molecular Weight: 857.93
MDL Number: MFCD00036314
Linear Formula: C47H52N7O7P
Product Type: Chemical
assay | ≥99.5% (reversed phase HPLC) |
>99.5% (31P-NMR) | |
biological source | non-animal source (no BSE/TSE risk) |
color | white to off-white |
form | powder or granules |
impurities | >= 170ppm, none detected (S/N <2.5) |
≤0.1% P(III) 100-169ppm (31P-NMR) | |
≤0.1% single unspecified Imprity (reversed phase HPLC) | |
≤0.3% dA2 (reversed phase HPLC, DMT-dA(bz) cyanoethyl-H-posphonate) | |
≤0.3% dA3 (reversed phase HPLC, DMT-dA(bz) phosphoramidate) | |
≤0.30 wt. % water content (Karl Fischer) | |
≤0.5% P(V) -25-99ppm (31P-NMR) | |
≤3.0 wt. % residual solvent content | |
InChI | 1S/C47H52N7O7P/c1-32(2)54 |
InChI key | GGDNKEQZFSTIMJ-AKWFTNRHSA |
nucleoside profile | base: deoxyadenosine base protecting group: benzoyl 2' protecting group: none 5' protecting group: DMT deprotection: standard |
product line | Proligo Reagents |
Quality Level | 300 |
SMILES string | COc1ccc(cc1)C(OC[C@H]2O[C |
solubility | soluble, clear |
storage temp. | 2-8°C |
suitability | conforms to structure for H-NMR |
conforms to structure for LC-MS | |
technique(s) | oligo synthesis: suitable |
type | for DNA synthesis |
Application: | Pharmadite products fulfill all requirements of starting materials for themanufacture of active pharmaceutical ingredients as defined in the EMEA "Note for guidance on the chemistry of the new active substance," making them suitable building blocks for oligonucleotide drugs. |
General description: | DMT-dA(bz) Pharmadite® is a Pharmadite® product line that represents a new class of standard protected DNA phosphoramidites that are designed with highly controlled impurity profiles and exceptional overall purity. Pharmadite phosphoramidites are characterized by their exceptional purity and well-defined impurity profile. In particular, they are essentially free from contaminants that interfere in coupling reactions, such as other nucleosidic or non-nucleosidic phosphoramidites (P(III)-contaminants). All remaining impurities in Pharmadite phosphopramidites, if present, are identified, characterized, and quantified. The impact of any such defined impurities on the synthesis of oligonucleotides is well understood and has been shown to be insignificant. |
Legal Information: | Pharmadite is a registered trademark of Merck KGaA, Darmstadt, Germany |
Other Notes: | Pharmadite phosphoramidites are manufactured in scales of up to multi-hundred kilos, under certified ISO 9001 quality systems. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.5% (reversed phase HPLC); >99.5% (31P-NMR) |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |