Anti-Actopaxin antibody produced in rabbit
SIGMA/A1226 - IgG fraction of antiserum, PBS solution
Synonym: Anti-α-Parvin; Anti-CH-ILKBP
MDL Number: MFCD04040144
Product Type: Chemical
Western Blotting Whole cell extract of Human endothelial ECV304 cell line were separated on SDS-PAGE and probed with Anti-Actopaxin antibody produced in Rabbit (Cat. No. A1226). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:3,000 antibody 2. 1:3,000 antibody + inhibition with immunizing peptide
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | PBS solution |
| mol wt | antigen 42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:3,000 using whole cell extract of the human endothelial ECV304 cell line. | |
| UniProt accession no. | Q9NVD7  |
| Application: | Actopaxin was detected in HEK 293 cell lysates at 42 kDa by western blot using rabbit anti-actopaxin antibody at a 1:1000 dilution. In these cells, actopaxin associated with an ILK complex. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Rabbit anti-actopaxin can also be used for protein microaarays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actopaxin is a member of actin binding proteins that include beta-parvin/affixin and gamma-parvin. Actopaxin is known to associate with F-actin and paxillin LD motifs LD1 and LD4. This protein also directly binds to serine/threonine integrin-linked kinase (ILK). Studies have reported that actopaxin may be involved in integrin-dependent remodeling of the actin cytoskeleton during cell adhesion, motility and division. Rabbit anti-actopaxin recognizes actopaxin (42 kDa). Staining of actopaxin in immunoblotting is specifically inhibited with actopaxin immunizing peptide (mouse, amino acids 35-53). |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of mouse actopaxin (amino acids 35-53). The sequence is identical in human actopaxin and has considerable homology (~70%) with β-parvin/affixin, but no homology with γ-parvin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |