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ABTS Enhancer

SIGMA/A1227 - for peroxidase detection

Synonym: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-A1227-10ML 10 mL
 $93.30
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form liquid
Quality Level 100 
storage temp. 2-8°C
Application: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor. It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.
Biochem/physiol Actions: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate. It might exhibit antioxidant property. The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.
Physical form: Supplied as an 11X concentrate
Storage Temp. 2-8°C
UNSPSC 12352204

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