Anti-Aurora-A Kinase antibody, Mouse monoclonal
SIGMA/A1231 - clone 35C1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Aurora-A Kinase; Monoclonal Anti-Aurora-A Kinase antibody produced in mouse; Anti-ARK-1; Anti-AURORA2; Anti-Aurora-related kinase 1; Anti-BTAK; Anti-STK15; Anti-STK6; Anti-
MDL Number: MFCD06411780
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Aurora-A Kinase Clone: 35C1 (Cat. No. A1231). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. HEK-293T 3. Jurkat 4. MM3MG 5. EL4
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 35C1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | PBS solution |
| isotype | IgG2b |
| mol wt | antigen ~46 kDa |
| Quality Level | 200  |
| recombinant | expressed in mouse cell line |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.25-0.5 μg/mL using HeLa total cell extract | |
| UniProt accession no. | O14965  |
| Application: | Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry. |
| Application: | Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation. |
| Biochem/physiol Actions: | Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans . |
| Biochem/physiol Actions: | Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome. |
| Immunogen: | recombinant human Aurora-A kinase. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Specificity: | Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |