AMPK (α1/β1/γ1), active, His tagged human
SIGMA/A1233 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: AMPKG; HAMPKb; MGC17785; MGC33776; MGC57364; MGC8666; PRKAA1; PRKAB1; PRKAG1
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | A1 subunit ~68 kDa |
B1 subunit ~38 kDa | |
G1 subunit ~40 kDa | |
product line | PRECISIO® Kinase |
Quality Level | 200 |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 626-848 nmol/min·mg |
storage temp. | −70°C |
UniProt accession no. | P54619 |
Q13131 | |
Q9Y478 |
Application: | AMP-activated protein kinase (AMPK) has been used to access the binding kinetics of thalidezine to recombinant active human AMPK using Bio-Layer Interferometry (BLI) binding assay. |
Biochem/physiol Actions: | AMP-activated protein kinase (AMPK) is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and 3-hydroxy-3-methylglutary |
General description: | Research area: CELL SIGNALING AMP-activated protein kinase (AMPK) is a heterotrimer protein kinase consisting of a α catalytic subunit, and non-catalytic β and γ subunits. |
Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol. |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H360D |
Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 626-848 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |