Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
SIGMA/A1293 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)–AP
MDL Number: MFCD00162620
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:100 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1:1,000 Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:30,000 antibody 2. 1:60,000 antibody 3. Negative Control
ELISA Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293) was serially diluted to detect Mouse IgG by direct ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| recombinant | expressed in goat |
| shipped in | wet ice |
| should not react with | human |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| western blot (chemiluminescent): 1:30,000 |
| Application: | Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm. |
| Application: | Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in: • enzyme-linked immunosorbent assay (ELISA){47 • immunohistochemistry • western blot |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . |
| Immunogen: | Purified mouse IgG Fab fragment |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| Specificity: | Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |