Anti-ATP6AP1 (451-465) antibody produced in rabbit
SIGMA/A1486 - IgG fraction of antiserum, PBS solution
Synonym: Anti-16A; Anti-ATP6IP1; Anti-ATP6S1; Anti-Ac45; Anti-CF2; Anti-VATPS1; Anti-Vacuolar ATP synthase subunit S1 precursor; Anti-XAP3
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells overexpressing human ATP6AP1 was separated on SDS-PAGE, blotted with Anti-ATP6AP1 (451-465) (Cat. No. A1486) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250
Immunofluorescence Anti-ATP6AP1 (451-465): Cat. No. A1486: Immunofluorescence of HUVEC cells using ATP6AP1 (451-465), Cat. No. A1486 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Chart Anti-ATP6AP1 (451-465): Cat. No. A1486: Intensity analysis of Anti-ATP6AP1 (451-465) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | PBS solution |
| mol wt | antigen ~52 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2,000 |
| UniProt accession no. | Q15904  |
| Application: | Rabbit anti-ATP6AP1 (451-465) antibody can be used for western blot (1:500-1:2,000) assays. |
| Application: | Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | ATPase H+ transporting accessory protein 1 (ATP6AP1) is known to cause an X-linked N-glycosylation syndrome with liver disease. It is essential for endosomal acidification. It also participates in membrane trafficking and Ca2+-dependent membrane fusion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATPase H+ transporting accessory protein 1 (ATP6AP1) is an accessory subunit of the V-ATPase. This gene is located on human chromosome Xq28. ATP6AP1 is also referred as Ac45. It is abundantly expressed a high levels in neuronal and (neuro-) endocrine cells and osteoclasts. |
| Immunogen: | synthetic peptide corresponding to amino acids 451-465 of human ATP6AP1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |