α-Amylase Inhibitor from Triticum aestivum (wheat seed)
SIGMA/A1520 - Type I, lyophilized powder
MDL Number: MFCD00130475
Product Type: Chemical
| biological source | Triticum estivum |
| composition | Protein, 35-65% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥1000 inhibitor U/mg protein (using human salivary α-amylase) |
| ≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase) | |
| storage temp. | −20°C |
| type | Type I |
| Application: | α-Amylase Inhibitor from Triticum aestivum (wheat seed) has been used to determine its effects on salivary gland (SG) amylase activity. It has also been used to inhibit the activity from the extract from Bemisia tabaci. |
| Biochem/physiol Actions: | Competitive inhibitor of human salivary α-amylase. KI = 2.9 nM, compared to a KM of 5.9 mM (calculated per mole of α-1,4-linked maltose residues). |
| Biochem/physiol Actions: | α-Amylases can hydrolyze α-(1,4)- |
| General description: | α-Amylases are endoglycosidases, that are classified into glycosyl hydrolase family 13 (1-3). |
| Packaging: | 1, 5, 25 mg in glass bottle |
| Physical form: | Lyophilized powder containing buffer salts as sodium phosphate. |
| Unit Definition: | One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1000 inhibitor U/mg protein (using human salivary α-amylase); specific activity: ≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |