Alginate Lyase
SIGMA/A1603 - powder, ≥10,000 units/g solid
Synonym: poly-
CAS Number: 9024-15-1
MDL Number: MFCD03454593
Product Type: Chemical
form | powder |
Quality Level | 200 |
specific activity | ≥10,000 units/g solid |
storage temp. | 2-8°C |
Application: | Alginate Lyase has been used: • in the pre-processing of enzyme • to de-encapsulate cells • to digest Pseudomonas exopolysaccharide to facilitate electroporation |
Application: | Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture . |
Biochem/physiol Actions: | Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol. |
Biochem/physiol Actions: | Breaks down alginate or alginic acid to smaller molecules and reduces viscosity. |
General description: | Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria. |
Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
Packaging: | 100 mg in poly bottle |
Preparation Note: | Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH. |
Unit Definition: | One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥10,000 units/g solid |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 4.2.2.3 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |