Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
SIGMA/A1682 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)–AP
MDL Number: MFCD00162620
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:40 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1682).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1682) Lanes 1. 1:20,000 antibody 2. 1:40,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | human, rat |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10 | |
| western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well) |
| Application: | Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm. |
| Application: | Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is suitable for use in western blot at 1:1000 dilution using human airway epithelial cells or using whole-cell extracts of rat tubal tissues . The antibody can also be used for immunohistochemistry (1:10 using formalin-fixed, paraffin-embedded sections). |
| Application: | Goat anti-mouse Fab specific alkaline phosphatase antibody was used to analyze serum Ig titers by His Sorb ELISA at 1:10000 at room temperature for two hours. Antibodies were diluted in PBS plus 0.2% BSA to reduce background. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody detects mouse IgG (whole molecule). When tested by ELISA, the product reacts with the Fab fragment of mouse IgG but does not recognize the Fc fragment of mouse IgG, human IgG, IgA, IgM or rat IgG. |
| Immunogen: | Purified mouse IgG |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 with 50% glycerol as stabilizer and 15 mM sodium azide |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |