G 418 disulfate salt

SIGMA/A1720 - powder, BioReagent, suitable for cell culture

Synonym: Antibiotic G418

CAS Number: 108321-42-2
Empirical Formula (Hill Notation): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Molecular Weight: 692.71
MDL Number: MFCD00058314
Linear Formula: C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A1720-1G	1 g	$142.00 1/EA	Add To Favorites
45-A1720-5G	5 g	$483.00 1/EA	Add To Favorites
45-A1720-25G	25 g	$2100.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A1720-1G.

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	Gram-negative bacteria
color	white to off-white
form	powder
InChI	1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;21-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key	UHEPSJJJMTWUCP-NKCAIAFTSA-N
mode of action	protein synthesis \| interferes
mol wt	692.7
optical activity	[α]/D (Specific Rotation: +104.4^o(c=0.3% in H₂O at 26^oC))
packaging	pkg of 1 g
	pkg of 25 g
	pkg of 5 g
potency	≥720 μg per mg (Dried basis)
product line	BioReagent
Quality Level	200
SMILES string	OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
solubility	H₂O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.
Biochem/physiol Actions:	G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.
Caution:	The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.
General description:	Chemical structure: aminoglycoside
General description:	G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.
Other Notes:	Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Packaging:	1, 5, 25 g in glass bottle
Preparation Note:	G418 powder is soluble in water at 100 mg/mL.

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334
Precautionary statements	P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	22-36/37-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Storage Temp.	2-8°C
UNSPSC	12352207

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