Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse
SIGMA/A1812 - clone FL-D6, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-FITC
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:250 Monoclonal Anti-Actin, α-Smooth Muscle-FITC antibody produced in Mouse, Clone: 1A4 (Cat. No. F3777) followed by 1:100 Monoclonal Anti-FITC-Alkaline Phosphatase antibody produced in Mouse, Clone: FL-D6 (Cat. No. A1812).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FL-D6, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| recombinant | expressed in mouse cell line |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate | |
| indirect ELISA: 1:30,000 |
| Application: | Alkaline phosphatase-conjugated mouse anti-FITC antibody was used for immunohistochemistry on mouse spleens. Tissue sections were fixed in acetone, blocked with PBS/5% goat serum/ 0.1% Tween and incubated with a FITC-conjugated primary antibody prior to the addition of the AP-conjugated anti-FITC antibody. |
| Application: | Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent such as alkaline phosphatase or horseradish peroxidase. Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It can be used in ELISA and immunofluorescent techniques. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | FITC-BSA conjugate |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide |
| Specificity: | The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |