2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

SIGMA/A1888 - ≥98% (HPLC)

Synonym: AzBTS-(NH₄)₂; Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

CAS Number: 30931-67-0
Empirical Formula (Hill Notation): C₁₈H₂₄N₆O₆S₄
Molecular Weight: 548.68
EC Number: 250-396-6
MDL Number: MFCD00010404
Linear Formula: C₁₈H₂₄N₆O₆S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A1888-1G	1 g	$88.70 1/EA	Add To Favorites
45-A1888-2G	2 g	$120.00 1/EA	Add To Favorites
45-A1888-5G	5 g	$309.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
form	powder
InChI	1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key	OHDRQQURAXLVGJ-AXMZSLBLSA-N
Quality Level	200
SMILES string	[NH4+].[NH4+].CCN1C(Sc2cc(ccc12)S([O-])(=O)=O)=NN=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
solubility	water: 10 mg/mL, clear to slightly hazy
storage temp.	2-8°C

Application:	• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay). • ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods. • ABTS is also used to monitor glucose concentrations in different solutions including blood serum. • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results.
Application:	2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
General description:	ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate: • ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example). • ABTS together with laccase or bilirubin oxidase. ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures. A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.
Packaging:	1, 2, 5 g in poly bottle

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Purity	≥98% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12352204

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