Asialofetuin from fetal calf serum
SIGMA/A1908 - Type II (Sigma designation)
MDL Number: MFCD00130561
Product Type: Chemical
biological source | bovine |
form | powder |
impurities | salt, essentially free |
≤1.0% N-acetylneuraminic acid | |
Quality Level | 200 |
solubility | H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow |
storage temp. | 2-8°C |
technique(s) | activity assay: suitable |
inhibition assay: suitable | |
type | Type II (Sigma designation) |
Application: | Asialofetuin from fetal calf serum is suitable for use: • in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose • in the inhibition of zona pellucida hardening of mouse oocytes • as an acceptor in sialyltransferase enzyme activity assay |
Application: | Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy. |
Biochem/physiol Actions: | Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells. |
Biochem/physiol Actions: | Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines. |
Packaging: | 50 mg in poly bottle |
Preparation Note: | Enzymatic cleavage using neuraminidase |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |