Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
SIGMA/A1949 - clone RG-96, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Rabbit IgG (γ-chain specific)
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:200 Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal (Cat. No. A1949).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal, Clone: RG-96 (Cat. No. A1949). Lanes 1. 1:160,000 antibody 2. 1:320,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-96, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| should not react with | goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 | |
| western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well) |
| Application: | Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in • various immunochemical assays like enzyme linked immunosorbent assay (ELISA) • westernblotting • immunohistology • dot immunobinding assay |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. |
| General description: | Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications. |
| General description: | The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep. |
| Immunogen: | Rabbit IgG |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |