Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
SIGMA/A1970 - clone P5D4, purified from hybridoma cell culture, PBS suspension
Synonym: Monoclonal Anti-VSV Glycoprotein
Product Type: Chemical
| analyte chemical class(es) | proteins (VSV-G) |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| capacity | ≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein) |
| clone | P5D4, monoclonal |
| conjugate | agarose conjugate |
| form | PBS suspension |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| protein purification: suitable |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) |
| Application: | For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. |
| General description: | Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose. |
| Immunogen: | synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH. |
| Physical form: | Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative |
| Preparation Note: | Prepared using cyanogen bromide-activated agarose. |
| Specificity: | The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |