Anti-β-Actin antibody, Mouse monoclonal
SIGMA/A1978 - clone AC-15, purified from hybridoma cell culture
Synonym: Monoclonal Anti-β-Actin
Product Type: Chemical
Western Blotting JOURNAL CITATION: Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor. By: Li, H., Liu, P., et al. in J Inflamm (Lond), 2008. PubMed ID: 18808711 Image collected and cropped by CiteAb from the following publication, (http://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-5-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor. By: Li, H., Liu, P., et al. in J Inflamm (Lond), 2008. PubMed ID: 18808711 Image collected and cropped by CiteAb from the following publication, (http://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-5-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor. By: Li, H., Liu, P., et al. in J Inflamm (Lond), 2008. PubMed ID: 18808711 Image collected and cropped by CiteAb from the following publication, (http://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-5-15), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 10 μg/mL Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A1978) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1 μg/mL Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A1978). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence CFB cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1 μg/mL Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A1978). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-β-Actin Clone: AC-15 (Cat. No. A1978). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | AC-15, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 42 kDa |
| Quality Level | 300  |
| shipped in | dry ice |
| should not react with | Dictyostelium discoideum |
| species reactivity | sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 10-40 μg/mL using human foreskin fibroblasts |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts. | |
| UniProt accession no. | P60709  |
| Application: | Monoclonal anti-beta-actin antibody can be used for microarray, indirect immunofluorescence, and immunohistochemical analyses. Furthermore, the product has been used for immunocytochemistry at 10-40 μg/mL using human foreskin fibroblasts. The antibody has also been used for western blot at 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. |
| Application: | Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates. |
| Application: | The actin in cells of various species and tissue origin is very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera to this protein. Therefore the availability of monoclonal antibodies to β-actin provides a specific and useful tool in studying the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton. Monoclonal anti-beta-actin antibody can be used for microarray, indirect immunofluorescence, and immunohistochemical analyses. Furthermore, the product has been used for immunocytochemistry at 10-40 μg/mL using human foreskin fibroblasts. The antibody has also been used for western blot at 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. It has been used in Immunoblot analysis. |
| Biochem/physiol Actions: | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. ACTB (β-actin) is crucial for organogenesis, especially for the development of brain, kidney and heart. It is responsible for maintaining cell proliferation, migration and shape. Actin protein is essential for the formation of mature platelets. β−Actin plays a key role in embryonic development. Upregulation of β-actin stimulates membrane protrusions and helps in cellular motility. The encoded protein β-Actin mediates fibroblast migration in mouse and thereby control connective tissue tension. Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| General description: | The ACTB (β-actin) gene is mapped to human chromosome 7p22.1. β-Actin is the most abundant protein localized to the cytoplasm. ACTB is expressed ubiquitously. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds. Four of the actin isoforms represent the differentiation markers of muscle tissues and two are found in almost all cells. Anti-β-Actin antibody, Mouse Monoclonal (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a slightly modified synthetic b-cytoplasmic actin N-terminal peptide Ac-Asp-Asp-Asp-Ile-Ala-Al In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| Immunogen: | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Storage and Stability: For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | Anti-β-Actin antibody, Mouse Monoclonal 1, 2 recognizes an epitope located on the N-terminal end of the β-isoform of actin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |