ACCUSPIN™ Tubes Sterile, 50 mL Capacity
SIGMA/A2055 - radiation sterilized tube fitted with a high density polyethylene barrier
Product Type: Chemical
ACCUSPIN™ Tubes Sterile is suitable for use in hematological and histological applications. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| application(s) | hematology histology |
| Quality Level | 200  |
| sterility | sterile; irradiated |
| storage temp. | 15-25°C |
| Application: | ACCUSPIN™ Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077: • in the isolation of lymphocytes and other mononuclear cells. • isolation of cord blood–derived endothelial progenitor cells. • isolate mononuclear cell layer from bone marrow samples. • suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia. |
| General description: | ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient. |
| Legal Information: | Accuspin is a trademark of Sigma-Aldrich Co. LLC |
| Principle: | Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077int |
| Quantity: | Package contains 10 tubes |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 15-25°C |
| UNSPSC | 12352207 |