Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal
SIGMA/A2064 - clone GG-5, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG (γ-chain specific)
Product Type: Chemical
ELISA Anti-Human IgG-Alkaline Phosphatase antibody, Mouse monoclonal, Clone: GG-5 (Cat. No. A2064) was serially diluted to detect Human IgG by direct ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GG-5, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:50,000 |
| dot blot: 1:80,000 | |
| immunohistochemistry: suitable | |
| western blot (chemiluminescent): 1:80,000 |
| Application: | Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in: • enzyme linked immunosorbent assay (ELISA) • western blotting • dot blot • immunohistology |
| Application: | Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. |
| General description: | Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Physical form: | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |