Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
SIGMA/A2074 - clone RG-16, purified immunoglobulin, lyophilized powder
Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:100 Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in Mouse, Clone: RG-16 (Cat. No. A2074).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1:1,000 Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in Mouse, Clone: RG-16 (Cat. No. A2074). Lanes 1. 1:160,000 antibody 2. 1:320,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-16, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| should not react with | pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue | |
| western blot (chemiluminescent): 1:160,000 |
| Application: | Monoclonal Anti-Rabbit Immunoglobulins-Peroxidas • western blotting • immunoblotting • immunoprecipitation • enzyme linked immunosorbent assay (ELISA) • immunohistochemistry |
| Application: | Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue). |
| Application: | The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. |
| General description: | Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM. |
| Physical form: | Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |