Anti-Actin, N-terminal antibody produced in rabbit
SIGMA/A2103 - ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00145889
Product Type: Chemical
Western Blotting JOURNAL CITATION: V600EBraf induces gastrointestinal crypt senescence and promotes tumour progression through enhanced CpG methylation of p16INK4a. By: Carragher, L. A., Snell, K. R., et al. in EMBO Mol Med, 2010. PubMed ID: 20941790 Image collected and cropped by CiteAb from the following publication, (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201000099), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: V600EBraf induces gastrointestinal crypt senescence and promotes tumour progression through enhanced CpG methylation of p16INK4a. By: Carragher, L. A., Snell, K. R., et al. in EMBO Mol Med, 2010. PubMed ID: 20941790 Image collected and cropped by CiteAb from the following publication, (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201000099), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluorescence. CFB cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:40 Anti-Actin antibody produced in Rabbit (Cat. No. A2066) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887), and 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887). Results Two Anti-Actin antibodies, A2066 (A) and A2103 (B) target different regions of Actin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 2 μg/mL Rabbit Anti-Actin N-terminal (Cat. No. A2103) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 1:50 Rabbit Anti-Actin (Cat. No. A2066) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence CFB cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 2 μg/mL Rabbit Anti-Actin N-terminal (Cat. No. A2103). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 ng/mL Rabbit Anti-Actin, N-terminal (Cat. No. A2103). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. P19 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 42 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | wet ice |
| species reactivity | frog, rat, mouse, chicken, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle | |
| indirect immunofluorescence: suitable | |
| western blot: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line. | |
| western blot: 2-4 ng/mL using whole extract of rat skeletal muscle | |
| UniProt accession no. | P63261  |
| Application: | Anti-Actin, N-terminal antibody produced in rabbit has been used in • immunoblotting • quantitative chromatin immunoprecipitation (qChIP) |
| Application: | Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa. |
| Application: | Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. |
| General description: | Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported. |
| Immunogen: | synthetic actin N-terminal nonapeptide conjugated to KLH. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |