Anti-AP Endonuclease antibody, Mouse monoclonal
SIGMA/A2105 - clone APEREF, purified from hybridoma cell culture
Synonym: Anti-AP lysase; Anti-APE/Ref1; Anti-APE1; Anti-APEN; Anti-APEX nuclease; Anti-APEX1; Anti-
MDL Number: MFCD01633033
Product Type: Chemical
Western Blotting Whole extract of Raji cells were separated on SDS-PAGE and probed with Anti-AP Endonuclease antibody, Mouse monoclonal, Clone: APEREF (Cat. No. A2105). The antibody was developed using 1:6,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 2 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | APEREF, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~37 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total cell extract of Raji cells | |
| UniProt accession no. | P27695  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Mouse monoclonal anti-AP endonuclease antibodies have been used for western blot analysis. The antibody can also be used for microarray, indirect ELISA and immunocytochemistry assays. |
| Biochem/physiol Actions: | AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) has a 3′-repair diesterase or phosphatase activity. The major DNA repair activity of the enzyme is nicking the DNA phosphodiester backbone 5′ to the AP site leaving a 3′-hydroxyl group and a 5′-deoxyribose phosphate. APE/Ref1 stimulates the DNA binding activity of many transcription factors (Fos, Jun, NF?B, PAX, HIF1, p53 and CREB), which are involved in cancer promotion and progression. The protein was found at elevated levels in several tumors (ovarian, prostate, cervical and germ cells). Redox-factor 1 (Ref1) maintains transcription factors in their active reduced states. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) is a crucial enzyme in the DNA base excision repair (BER) pathway. It recognizes baseless sites in the DNA following spontaneous base loss or removal of a damaged base by different DNA glycosylases. This enzyme is the major AP endonuclease in mammalian cells. The human enzyme shows a high degree of homology with other mammalian AP endonucleases (bovine, mouse, rat, and hamster). APE/Ref1 is a multifunctional enzyme. Its N-terminal region includes a nuclear localization sequence and a redox activity zone, while its AP endonuclease activity resides in the C-terminal region. Redox-factor 1 (Ref1) refers to the reduction/oxidation function of the enzyme. |
| General description: | Monoclonal Anti-AP Endonuclease recognizes human, canine, rat, and mouse AP endonuclease (approx. 37kDa). |
| General description: | The AP endonuclease is a DNA damage repair gene involved in base excision repair. |
| Immunogen: | recombinant human AP endonuclease. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |