DMT-2′O-Methyl-rA(bz) Phosphoramidite

SIGMA/A211140 - configured for MerMade

Synonym: DMT-2′-O-Me-rA(bz) amidite; N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-adenosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]

Empirical Formula (Hill Notation): C₄₈H₅₄N₇O₈P
Molecular Weight: 887.96
MDL Number: MFCD00792622
Linear Formula: C₄₈H₅₄N₇O₈P
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A211140-1G	1 g	$101.00 1/EA	Add To Favorites
45-A211140-5G	5 g	$909.00 1/EA	Add To Favorites
45-A211140-10G		No Price

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (³¹P-NMR)
	≥99.0% (redox titration)
biological source	non-animal source (no BSE/TSE risk)
color	white to off-white
compatibility	configured for MerMade
form	powder
impurities	≤0.3% mA2 (reversed phase HPLC, Hydrolysate)
	≤0.3% mA3 (reversed phase HPLC, DMT-rA(bz)me)
	≤0.3% water content (Karl Fischer)
	≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR)
	≤0.5% single Impurity (redox titration)
	≤1.0% mA1 (reversed phase HPLC, DMT-rA(bz)me-DMT)
	≤3% residual Solvent content
InChI	1S/C48H54N7O8P/c1-32(2)55(33(3)4)64(61-28-14-27-49)63-42-40(62-47(43(42)59-7)54-31-52-41-44(50-30-51-45(41)54)53-46(56)34-15-10-8-11-16-34)29-60-48(35-17-12-9-13-18-35,36-19-23-38(57-5)24-20-36)37-21-25-39(58-6)26-22-37/h8-13,15-26,30-33,40,42-43,47H,14,28-29H2,1-7H3,(H,50,51,53,56)/t40-,42-,43-,47-,64?/m1/s1
InChI key	AZCGOTUYEPXHMJ-PSVHYZMASA-N
product line	Proligo Reagents
Quality Level	300
SMILES string	CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6c(NC(=O)c7ccccc7)ncnc56
storage temp.	2-8°C
suitability	conforms to structure for H-NMR
	conforms to structure for LC-MS

Features and Benefits:	• High yield of crude oligonucleotides • Compatible with DNA synthesis • Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides • Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C • Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rA(bz) Phosphoramidite is configured for MerMade Synthesizers.
General description:	Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:• Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules
Other Notes:	The synthesis cycle for 2′O-Methyloligoribonucleotides consists of the sameseries of reactions as the cycle that is employed for DNA monomers.However, the rate of coupling for 2′O-Methyl RNA monomers is slowercompared to that of DNA monomers (a coupling time of 6 minutes isrecommended for 2′O-Methyl RNA monomers compared to 90 seconds forDNA monomers). With the exception of the 2′O-Methyl RNA monomers andsupports, RNA synthesis is accomplished with the same reagents as DNAsynthesis. All 2′O-Methyl RNA phosphoramidites from Sigma-Aldrich arediluted with dry acetonitrile.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (³¹P-NMR); ≥99.0% (redox titration)
Storage Temp.	2-8°C
UNSPSC	12352200

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