DMT-2′O-Methyl-rA(bz) Phosphoramidite
SIGMA/A211150 - configured for (ÄKTA® and OligoPilot®), configured for ABI
Synonym: DMT-2′-O-Me-rA(bz) amidite; N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-adenosine, 3′-[2-cyanoethyl N,N-
CAS Number: 110782-31-5
Empirical Formula (Hill Notation): C48H54N7O8P
Molecular Weight: 887.96
MDL Number: MFCD00792622
Linear Formula: C48H54N7O8P
Product Type: Chemical
| λ | conforms (UV/VIS Identity) |
| assay | ≥99% (31P-NMR) |
| ≥99.0% (reversed phase HPLC) | |
| biological source | non-animal source (no BSE/TSE risk) |
| color | white to off-white |
| compatibility | configured for (ÄKTA® and OligoPilot®) |
| configured for ABI | |
| form | powder |
| impurities | ≤0.1% single unspecified Impurity (reversed phase HPLC) |
| ≤0.3% mA2 (reversed phase HPLC, Hydrolysate) | |
| ≤0.3% mA3 (reversed phase HPLC, DMT-rA(bz)me) | |
| ≤0.3% water content (Karl Fischer) | |
| ≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
| ≤1.0% mA1 (reversed phase HPLC, DMT-rA(bz)me-DMT) | |
| ≤3% residual Solvent content | |
| InChI | 1S/C48H54N7O8P/c1-32(2)55 |
| InChI key | AZCGOTUYEPXHMJ-PSVHYZMASA |
| nucleoside profile | base: adenosine base protecting group: benzoyl 2' protecting group: methyl 5' protecting group: DMT deprotection: standard |
| product line | Proligo Reagents |
| Quality Level | 300  |
| SMILES string | CO[C@@H]1[C@H](OP(OCCC#N) |
| storage temp. | 2-8°C |
| suitability | conforms to structure for H-NMR |
| conforms to structure for LC-MS | |
| technique(s) | oligo synthesis: suitable |
| Application: | 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rA(bz) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: • Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules (a) |
| General description: | DMT-2′O-Methyl-rA(bz) Phosphoramidite is a 2′O-Methyl RNA monomer that is compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. Modern phosphoramidite-mediated synthesis has enabled routine high-yielding preparations of 2′O-Methyl RNA oligonucleotides. High-quality RNA phosphoramidites are key to low failure rates, the high biological activity of synthesis products, and cost-effectiveness. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complementary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | OligoPilot is a registered trademark of Cytiva |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |