DMT-2′Fluoro-dA(bz) Phosphoramidite
SIGMA/A211200
Synonym: DMT-2′Fluoro-dA(bz) Amidite
Empirical Formula (Hill Notation): C47H51FN7O7P
Molecular Weight: 875.92
MDL Number: MFCD12912392
Linear Formula: C47H51FN7O7P
Product Type: Chemical
λ | conforms (UV/VIS Identity) |
assay | ≥99% (31P-NMR) |
≥99.0% (reversed phase HPLC) | |
biological source | non-animal source (no BSE/TSE risk) |
color | white to off-white |
form | powder |
impurities | ≤0.3% water content (Karl Fischer) |
≤0.5% P(III) Impurities 100-169ppm (31P-NMR) | |
≤0.5% single Impurity (reversed phase HPLC) | |
≤3% residual Solvent content | |
InChI | 1S/C47H51FN7O7P/c1-31(2)5 |
InChI key | VCCMVPDSLHFCBB-MSIRFHFKSA |
nucleoside profile | base: deoxyadenosine base protecting group: benzoyl 2' protecting group: fluoro 5' protecting group: DMT deprotection: fast/standard |
product line | Proligo Reagents |
Quality Level | 300 |
SMILES string | CC(C)N(C(C)C)P(OCCC#N)O[C |
storage temp. | −20°C |
suitability | conforms to structure for H-NMR |
conforms to structure for LC-MS | |
technique(s) | oligo synthesis: suitable |
General description: | DMT-2′Fluoro-dA(bz) Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance. Its key features include, • Can be employed together with DNA or RNA phosphoramidites • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% (31P-NMR); ≥99.0% (reversed phase HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |