DMT-2′Fluoro-dA(bz) Phosphoramidite

SIGMA/A211250 - configured for (ÄKTA^® and OligoPilot^®)

Synonym: DMT-2′Fluoro-dA(bz) Amidite

Empirical Formula (Hill Notation): C₄₇H₅₁FN₇O₇P
Molecular Weight: 875.92
MDL Number: MFCD12912392
Linear Formula: C₄₇H₅₁FN₇O₇P
Product Type: Chemical

λ	conforms (UV/VIS Identity)
assay	≥99% (³¹P-NMR)
	≥99.0% (reversed phase HPLC)
biological source	non-animal source (no BSE/TSE risk)
color	white to off-white
compatibility	configured for (ÄKTA^® and OligoPilot^®)
form	powder
impurities	≤0.3% water content (Karl Fischer)
	≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR)
	≤0.5% single Impurity (reversed phase HPLC)
	≤3% residual Solvent content
InChI	1S/C47H51FN7O7P/c1-31(2)55(32(3)4)63(60-27-13-26-49)62-42-39(61-46(40(42)48)54-30-52-41-43(50-29-51-44(41)54)53-45(56)33-14-9-7-10-15-33)28-59-47(34-16-11-8-12-17-34,35-18-22-37(57-5)23-19-35)36-20-24-38(58-6)25-21-36/h7-12,14-25,29-32,39-40,42,46H,13,27-28H2,1-6H3,(H,50,51,53,56)/t39-,40-,42-,46-,63?/m1/s1
InChI key	VCCMVPDSLHFCBB-MSIRFHFKSA-N
nucleoside profile	base: deoxyadenosine base protecting group: benzoyl 2' protecting group: fluoro 5' protecting group: DMT deprotection: fast/standard
product line	Proligo Reagents
Quality Level	300
SMILES string	CC(C)N(C(C)C)P(OCCC#N)O[C@H]([C@H](O1)COC(C2=CC=C(OC)C=C2)(C3=CC=C(OC)C=C3)C4=CC=CC=C4)[C@@H](F)[C@@H]1N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5
storage temp.	−20°C
suitability	conforms to structure for H-NMR
	conforms to structure for LC-MS
technique(s)	oligo synthesis: suitable

Application:	DMT-2′Fluoro-dA(bz) Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.
General description:	DMT-2′Fluoro-dA(bz) Phosphoramidite is a 2′Fluoro phosphoramidite used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.Its key features are-• Can be employed together with DNA or RNA phosphoramidites • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system
Legal Information:	ÄKTA is a registered trademark of Cytiva
Legal Information:	OligoPilot is a registered trademark of Cytiva

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable