Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse
SIGMA/A2172 - clone 5C5, ascites fluid
Synonym: Mouse Anti-Actin
MDL Number: MFCD00164521
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. By: Ferro, F., Spelat, R., et al. in PLoS One, 2012. PubMed ID: 22844522 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0041774), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: BEX1 is an RNA-dependent mediator of cardiomyopathy. By: Accornero, F., Schips, T. G., et al. in Nat Commun, 2017. PubMed ID: 29192139 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-017-02005-1), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence
Formalin-fixed, paraffin-embedded human tongue tissue stained with Monoclonal Anti-α-Sarcomeric Actin, Clone No. 5C5 (Cat. No. A2172).
Formalin-fixed, paraffin-embedded human tongue tissue stained with Monoclonal Anti-α-Sarcomeric Actin, Clone No. 5C5 (Cat. No. A2172).
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tongue sections with 1:1,000 Monoclonal Anti-Actin (α-Sarcomeric) Clone: 5C5 (Cat. No. A2172) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:aceton. Fixed cells were stained with 1:500 Monoclonal Anti-Actin (α-Sarcomeric) Clone: 5C5 (Cat. No. A2172). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Actin (α-Sarcomeric) Clone: 5C5 (Cat. No. A2172). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Differentiated C2C12 2. Rat heart
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5C5, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human skeletal and cardiac muscle |
| immunohistochemistry (frozen sections): suitable using human skeletal and cardiac muscle | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P68032  |
| P68133 |
| Application: | Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has also been used • immunofluorescence • double immunostaining • double labelling terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) immunofluorescenc |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. |
| Application: | Mouse monoclonal anti-actin (alpha-sarcomeric) antibody has been used for western blot, and immunohistochemistry applications. |
| Biochem/physiol Actions: | Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-α-Sarcomeric Actin (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin is one of the most conserved eukaryotic proteins and the isoforms show >90% overall sequence homology. It is expressed in mammals and birds as six isoforms. Four of the six represent differentiation markers of muscle tissues. The other two are found in practically all cells. |
| Immunogen: | rabbit striated muscle actin. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | The product is provided as ascites fluid containing 0.1% sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |