Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat
SIGMA/A2179 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)–AP
MDL Number: MFCD00162620
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547), followed by 1:25 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179).
Immunohistochemistry Formalin fixed, paraffin-embedded, Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547), followed by 1:25 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-β-Actin antibody, Mouse monoclonal (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative Control
ELISA 10 μg/mL Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179), was probed to detect Mouse IgG by indirect ELISA. Reactivity is observed with Mouse IgG and Mouse Fab but not with Mouse Fc, Human IgG, Human IgM, Human IgA, Bovine IgG, Bovine IgM and Horse IgG.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | horse, human, bovine |
| species reactivity | mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 | |
| western blot (chemiluminescent): 1:80,000 |
| Application: | Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm. |
| Application: | Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in goat has been used in: • western blotting • immunoblotting • enzyme linked immunosorbent assay (ELISA) • immunohistochemistry |
| Biochem/physiol Actions: | Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises of the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc are is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and Staphylococcus protein A binding. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains held together by disulfide linkages. |
| Immunogen: | Purified mouse IgG, Fab fragment |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse, or human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |