ANTI-FLAG® M2 Affinity Gel
SIGMA/A2220 - purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal ANTI-FLAG® M2 antibody produced in mouse; ANTI-FLAG® M2 Affinity Agarose Gel; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| analyte chemical class(es) | proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag) |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| capacity | >0.6 mg/mL, resin binding capacity (FLAG-BAP) |
| clone | M2, monoclonal |
| conjugate | agarose conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| matrix | (4% agarose bead; 45-165μm bead size) |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide) |
| immunoprecipitation (IP): suitable |
| Application: | Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins. Learn more product details in our FLAG® application portal. |
| General description: | Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent. Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide |
| Immunogen: | DYKDDDDK |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Suspension in buffered saline containing azide as preservative and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |