Monoclonal Anti-β-Actin antibody produced in mouse
SIGMA/A2228 - clone AC-74, purified immunoglobulin, buffered aqueous solution
Synonym: Actin Antibody Sigma; Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse; Beta Actin Antibody Sigma; Beta Actin Sigma Antibody; Sigma Actin Antibody; Sigma Beta Actin Antibody
MDL Number: MFCD00164531
Product Type: Chemical
Western Blotting JOURNAL CITATION: Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation. By: Tao, Y., Xi, S., et al. in PLoS One, 2010. PubMed ID: 20161795 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0009163), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Negative regulation of bone formation by the transmembrane Wnt antagonist Kremen-2. By: Schulze, J., Seitz, S., et al. in PLoS One, 2010. PubMed ID: 20436912 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0010309), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation. By: Tao, Y., Xi, S., et al. in PLoS One, 2010. PubMed ID: 20161795 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0009163), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A2228). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 10 μg/mL Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A2228) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1 μg/mL Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A2228). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence HS-68 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 4 μg/mL Monoclonal Anti-β-Actin Clone: AC-74 (Cat. No. A2228). The antibody was developed using Goat Anti-Mouse IgG, FITC conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | AC-74, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 42 kDa |
| Quality Level | 300  |
| shipped in | dry ice |
| should not react with | Dictyostelium discoideum |
| species reactivity | rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts | |
| UniProt accession no. | P60709  |
| Application: | Monoclonal anti-β-actin antibody can be used for immunocytochemistry (10-40μg/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis. |
| Application: | Monoclonal Anti-β-Actin antibody has been used in western blot and two-dimensional gel immunoblot. |
| Application: | Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures. |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. |
| General description: | Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting. |
| Immunogen: | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Storage and Stability: | Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |