Apyrase from potatoes
SIGMA/A2230 - High Activity, ATPase ≥600 units/mg protein, lyophilized powder
Synonym: Adenosine 5′-diphosphatase; Adenosine 5′-triphosphatase
CAS Number: 9000-95-7
MDL Number: MFCD00130542
Product Type: Chemical
ATPase activity | ≥600 units/mg protein |
biological source | potato |
composition | protein, ≥30% |
foreign activity | Acid Phosphatase ≤2% of base activity |
form | lyophilized powder |
quality | High Activity |
Quality Level | 300 |
secondary activity | ≥50 % of base activity ADPase |
shipped in | wet ice |
storage temp. | −20°C |
Application: | Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase. |
Application: | At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. |
Biochem/physiol Actions: | Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. |
Packaging: | 100 units in glass bottle |
Packaging: | Sold on the basis of ATPase units. |
Physical form: | Lyophilized powder containing potassium succinate buffer salts. |
Preparation Note: | Derived from red potato |
Unit Definition: | One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | ATPase activity: ≥600 units/mg protein; secondary activity: ≥50 % of base activity ADPase |
Storage Temp. | −20°C |
Enzyme Commission (EC) Number | 3.6.1.5 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |