Anti-Axin1 (C-terminal region) antibody produced in rabbit
SIGMA/A2231 - ~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Axin; Anti-Axis Inhibitor 1; Anti-Axis inhibition protein 1
MDL Number: MFCD02095870
Product Type: Chemical
Immunoblotting Cell lysates of HEK-293T transfected with Mouse Axin1-FLAG expression vector, were separated on SDS-PAGE and blotted with Anti-Axin1 (C-terminal region) (Cat. No. A2231). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody dilution 1:5,000 2: Antibody dilution 1:5,000 +Inhibition with Axin1 immunizing peptide (human 815-833) (10 μg/mL) 3: Untransfected HEK-293T cells. Antibody dilution 1:5,000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~2.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1 | |
| UniProt accession no. | O15169  |
| Application: | Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons. |
| Application: | Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1μg/ml in western blot assays using HEK-293 cells expressing mouse axin1. |
| Biochem/physiol Actions: | Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC). |
| Biochem/physiol Actions: | Axin1 (axis inhibitor-1, axin) enhances the phosphorylation of β-catenin by Glycogen synthase kinase 3 β (GSK-3b). Axin1 is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3b thus, facilitating β-catenin phosphorylation by GSK-3b. Axin1 itself is phosphorylated and its activity regulated by GSK-3b. Axin1 phosphorylation is reduced by Wnt signaling, leading to decreased affinity of interaction of axin1 and β-catenin. This results in the release of β-catenin from the degradation complex and increased stability of β-catenin in the cell. Axin1 is destabilized by Wnt signaling and its levels in the cell are reduced. Axin isoform axin2 (conductin, axil) is 44% identical to Axin1 and appears to play a similar role to Axin1 in the Wnt signaling pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Axin1 contains two conserved domains, an N-terminal regulator of G-protein signaling (RGS) domain, and a C-terminal DIX domain. The C-terminal region of axin1 is important for homodimerization, whereas the central region binds β-catenin and GSK-3b. |
| Immunogen: | synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2. |
| Physical form: | Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |