Phosphatase, Alkaline shrimp
SIGMA/A2237 - ≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
Synonym: Phosphatase, Alkaline from shrimp; Orthophosphoric-
CAS Number: 9001-78-9
MDL Number: MFCD00131849
Product Type: Chemical
| concentration | ≥900 DEA units/mL |
| foreign activity | RNAse, DNAse, none detected |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| recombinant | expressed in proprietary host |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret |
| Application: | Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products. |
| Biochem/physiol Actions: | Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages. |
| General description: | Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes. |
| Physical form: | solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2 |
| Unit Definition: | One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |