α-Amanitin

SIGMA/A2263 - from Amanita phalloides, ≥85% (HPLC), powder

CAS Number: 23109-05-9
Empirical Formula (Hill Notation): C₃₉H₅₄N₁₀O₁₄S
Molecular Weight: 918.97
EC Number: 245-432-2
MDL Number: MFCD00215842
Linear Formula: C₃₉H₅₄N₁₀O₁₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-A2263-1MG	1 mg	$375.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε (extinction coefficient)	13,500 at 310 nm in H₂O at 1 M
assay	≥85% (HPLC)
biological source	Amanita phalloides
color	white to light yellow
form	powder
InChI	1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)
InChI key	CIORWBWIBBPXCG-UHFFFAOYSA-N
mol wt	918.97 g/mol
mp	254-255 °C (lit.)
Quality Level	200
SMILES string	CCC(C)C1NC(=O)CNC(=O)C2Cc3c([nH]c4cc(O)ccc34)S(=O)CC(NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)N5CC(O)CC5C(=O)NC(C(C)C(O)CO)C(=O)N2
solubility	H₂O: 1.0 mg/mL
storage temp.	−20°C

Amino Acid Sequence:	Asn-Hyp-Hyi-Trp-Gly-Ile-Gly-Cys [Bridge: 4-8]
Application:	α-Amanitin has been used: • for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay • to induce nephrotoxicity in mice renal tissues • to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay
Biochem/physiol Actions:	Inhibits eukaryotic RNA polymerase II and III; does not inhibit RNA polymerase I or bacterial RNA polymerase; inhibits mammalian protein synthesis.
Biochem/physiol Actions:	The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.
Disclaimer:	When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years.
Preparation Note:	Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.

Symbol	GHS06,GHS08
Signal word	Danger
Hazard statements	H300 - H373
Precautionary statements	P260 - P264 - P270 - P301 + P310 - P314 - P405
Hazard Codes	T+
Risk Statements	26/27/28-33
Safety Statements	36/37/39-45
RIDADR	UN 3462 6.1 / PGII
WGK Germany	WGK 3

Purity	≥85% (HPLC)
mp	254-255 °C (lit.)
Storage Temp.	−20°C
UNSPSC	12352202

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