α-Amanitin
SIGMA/A2263 - from Amanita phalloides, ≥85% (HPLC), powder
CAS Number: 23109-05-9
Empirical Formula (Hill Notation): C39H54N10O14S
Molecular Weight: 918.97
EC Number: 245-432-2
MDL Number: MFCD00215842
Linear Formula: C39H54N10O14S
Product Type: Chemical
ε (extinction coefficient) | 13,500 at 310 nm in H2O at 1 M |
assay | ≥85% (HPLC) |
biological source | Amanita phalloides |
color | white to light yellow |
form | powder |
InChI | 1S/C39H54N10O14S/c1-4-16( |
InChI key | CIORWBWIBBPXCG-UHFFFAOYSA |
mol wt | 918.97 g/mol |
mp | 254-255 °C (lit.) |
Quality Level | 200 |
SMILES string | CCC(C)C1NC(=O)CNC(=O)C2Cc |
solubility | H2O: 1.0 mg/mL |
storage temp. | −20°C |
Amino Acid Sequence: | Asn-Hyp-Hyi-Trp-Gly-Ile-G |
Application: | α-Amanitin has been used: • for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay • to induce nephrotoxicity in mice renal tissues • to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay |
Biochem/physiol Actions: | The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis. |
Caution: | When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years. |
Reconstitution: | Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base. |
Symbol | GHS06,GHS08 |
Signal word | Danger |
Hazard statements | H300 - H373 |
Precautionary statements | P260 - P264 - P270 - P301 + P310 - P314 - P405 |
Hazard Codes | T+ |
Risk Statements | 26/27/28-33 |
Safety Statements | 36/37/39-45 |
RIDADR | UN 3462 6.1 / PGII |
WGK Germany | WGK 3 |
Purity | ≥85% (HPLC) |
mp | 254-255 °C (lit.) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |