β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)

SIGMA/A2264 - ammonium sulfate suspension, ≥10 units/mg protein

Synonym: β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase

CAS Number: 9012-33-3
EC Number: 232-730-2
MDL Number: MFCD00081402
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A2264-5UN	5 units	$181.00 1/EA	Add To Favorites
45-A2264-10UN	10 units	$333.00 1/EA	Add To Favorites
45-A2264-25UN	25 units	$625.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

biological source	Canavalia ensiformis
foreign activity	α- and β-galactosidase, and α-L-fucosidase ≤0.1%
	α-mannosidase ≤0.2%
form	ammonium sulfate suspension
Quality Level	200
specific activity	≥10 units/mg protein
storage temp.	2-8°C

Analysis Note:	At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.
Application:	β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .
Biochem/physiol Actions:	This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
Biochem/physiol Actions:	This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Other Notes:	One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.
Physical form:	Suspension in 2.5 M (NH₄)₂SO₄, pH 7.0

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥10 units/mg protein
Storage Temp.	2-8°C
Enzyme Commission (EC) Number	3.2.1.52 ( BRENDA \| IUBMB )
UNSPSC	12352204

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