β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)
SIGMA/A2264 - ammonium sulfate suspension, ≥10 units/mg protein
Synonym: β-N-Acetyl-D-hexosaminide N-
CAS Number: 9012-33-3
EC Number: 232-730-2
MDL Number: MFCD00081402
Product Type: Chemical
biological source | Canavalia ensiformis |
foreign activity | α- and β-galactosidase, and α- |
α-mannosidase ≤0.2% | |
form | ammonium sulfate suspension |
Quality Level | 200 |
specific activity | ≥10 units/mg protein |
storage temp. | 2-8°C |
Analysis Note: | At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0. |
Application: | β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms . |
Biochem/physiol Actions: | This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. |
Biochem/physiol Actions: | This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β- |
Physical form: | Suspension in 2.5 M (NH4)2SO4, pH 7.0 |
Unit Definition: | One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β- |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥10 units/mg protein |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 3.2.1.52 ( BRENDA | IUBMB ) |
UNSPSC | 12352204 |