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Albumin, Tetramethylrhodamine isothiocyanate bovine

SIGMA/A2289 - lyophilized powder

Synonym: TRITC-albumin

CAS Number: 9048-46-8
MDL Number: MFCD00130416
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A2289-10MG 10 mg
$421.00
1/EA
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45-A2289-50MG 50 mg
$1690.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A2289-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A2289-50MG

 

assay ≥90% (Lowry)
biological source bovine
extent of labeling ≥0.5 mol TRITC per mol albumin
form lyophilized powder
impurities salt, free
Quality Level 200 
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
UniProt accession no. P02769 
Application: Albumin, Tetramethylrhodamine isothiocyanate has been used:
• in photoinduced release assay of polyethylene beads
• for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
• in fluorescent correlation spectroscopy

Application: Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
General description: Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).
Packaging: 10, 50 mg in poly bottle
Preparation Note: Prepared from crystallized and lyophilized albumin (A4378).
Purity ≥90% (Lowry)
Storage Temp. 2-8°C
UNSPSC 12352202

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