Albumin, Tetramethylrhodamine isothiocyanate bovine
SIGMA/A2289 - lyophilized powder
Synonym: TRITC-albumin
CAS Number: 9048-46-8
MDL Number: MFCD00130416
Product Type: Chemical
assay | ≥90% (Lowry) |
biological source | bovine |
extent of labeling | ≥0.5 mol TRITC per mol albumin |
form | lyophilized powder |
impurities | salt, free |
Quality Level | 200 |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P02769 |
Application: | Albumin, Tetramethylrhodamine isothiocyanate has been used: • in photoinduced release assay of polyethylene beads • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating • in fluorescent correlation spectroscopy |
Application: | Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy. |
General description: | Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC). |
Packaging: | 10, 50 mg in poly bottle |
Preparation Note: | Prepared from crystallized and lyophilized albumin (A4378). |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H302 + H312 + H332 - H315 - H319 - H334 - H335 |
Precautionary statements | P261 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 20/21/22-36/37/38-42 |
Safety Statements | 22-26-45 |
RIDADR | UN 3335 |
WGK Germany | WGK 3 |
Purity | ≥90% (Lowry) |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |