Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse
SIGMA/A2306 - clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:160 Monoclonal Anti-Rabbit Immunoglobulins-Alkaline Phosphatase antibody produced in Mouse, Clone: RG-16 (Cat. No. A2306).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Monoclonal Anti-Rabbit Immunoglobulins-Alkaline Phosphatase antibody produced in Mouse, Clone: RG-16 (Cat. No. A2306). Lanes 1. 160,000 antibody 2. 320,000 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-16, monoclonal |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| should not react with | pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody | |
| western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well) |
| Application: | Monoclonal Anti-Rabbit Immunoglobulins Alkaline Phosphatase antibody produced in mouse has been used in: • indirect enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunohistochemistry |
| Application: | The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature. |
| Biochem/physiol Actions: | IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody binds to an epitope on the heavy chain of rabbit IgG, IgA, and IgM. The product stains bands at the intact whole molecule and at the heavy chains in an immunoblot of denatured, non-reduced rabbit immunoglobulins. Reduction of rabbit immunoglobulins destroys the epitope. In ELISA, the product shows no cross reaction with human serum or tissue preparations, nor with human IgA, IgG, or IgM. |
| Biochem/physiol Actions: | The constant domains of immunoglobulins are involved in the complement activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins is composed of two heavy (H) and two light (L) chains. Each containing one NH2-terminal variable region and one or more COOH-terminal constant regions. They are heterodimeric proteins. |
| General description: | Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM. |
| Physical form: | Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
| Specificity: | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Specificity: | No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, sheep or turkey. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |