Phosphatase, Alkaline from bovine intestinal mucosa
SIGMA/A2356 - ≥5,500 DEA units/mg protein
Synonym: Alkaline phosphatase; Orthophosphoric-
CAS Number: 9001-78-9
MDL Number: MFCD00131849
Product Type: Chemical
One DEA unit will hydrolyze 1 micromole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
| concentration | ≥10 mg/mL |
| form | (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) |
| mol wt | dimer ~160 kDa |
| Quality Level | 200  |
| specific activity | ≥5,500 DEA units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Package sizes are based on DEA units |
| Application: | Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. |
| Application: | High specific activity grade recommended for antibody and protein conjugation. |
| Biochem/physiol Actions: | The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids. |
| Physical form: | Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6 |
| Physical properties: | Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer. |
| Physical properties: | Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity. |
| Unit Definition: | One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5,500 DEA units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |