Alcohol Oxidase solution from Pichia pastoris
SIGMA/A2404 - buffered aqueous solution, 10-40 units/mg protein (biuret)
Synonym: Alcohol:oxygen oxidoreductase
CAS Number: 9073-63-6
MDL Number: MFCD00130452
Product Type: Chemical
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | 10-40 units/mg protein (biuret) |
| storage temp. | −20°C |
| Application: | Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones . |
| Application: | Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples. |
| Biochem/physiol Actions: | Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well. |
| General description: | Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family. |
| Physical form: | Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0 |
| Unit Definition: | One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 10-40 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |