Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
SIGMA/A2554 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fc specific)–HRP
MDL Number: MFCD00162629
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human appendix sections stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by 1:200 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A2554).
Immunohistochemistry Formalin-fixed, paraffin-embedded human tonsil sections stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by 1:200 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A2554).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978). The antibody was developed using Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in Goat (Cat. No. A2554). Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| should not react with | bovine, human, horse |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:70,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 | |
| western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL) |
| Application: | Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in: • immunohistochemistry • enzyme-linked immunosorbent assay (ELISA) • immunoblot • dot-blot analysis |
| Application: | Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse, or human samples. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |